Phosphinothricin acetyltransferase gene

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August undefined, 2024

WebGlufosinate (also known as phosphinothricin and often sold as an ammonium salt) is a naturally occurring broad-spectrum herbicide produced by several species of … WebStrauch, E.; Wohlleben, W.; Puhler, A.: Cloning of a phosphinothricin Nacetyltransferase gene from Streptomyces viridochromogenes Tu494 and its expression in Streptomyces lividans and Escherichia coli. Gene, 63, 65–74 (1988) CrossRef CAS PubMed Google Scholar

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WebThe gene encodes phosphinothricin acetyltransferase, which N-acetylates phosphinothricin using acetyl-CoA. Figure 6.1 . Overview of biosynthetic pathways of a number of … Web10.1007/BF00201636 Abstract L-Phosphinothricin (L-Pt)-resistant plants were constructed by introducing a modified phosphinothricin-N-acetyl-transferase gene (pat) via … pool table parts spring

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WebIt codes for phosphinothricin acetyl transferase (PAT) proteins of 183 amino acids and shows 85% DNA sequence homology with another marker gene pat isolated from Streptomyces viridochromogenes. WebGene: pat Gene Source: Streptomyces viridochromogenes Product: phosphinothricin N-acetyltransferase (PAT) enzyme Function: eliminates herbicidal activity of glufosinate (phosphinothricin) herbicides by acetylation GM Trait: Glufosinate herbicide tolerance Events with gene pat Phosphinothricin acetyltransferase (EC 2.3.1.183, PAT, PPT acetyltransferase, Pt-N-acetyltransferase, ac-Pt) is an enzyme with systematic name acetyl-CoA:phosphinothricin N-acetyltransferase. This enzyme catalyses the following chemical reaction acetyl-CoA + phosphinothricin CoA + N-acetylphosphinothricin share doing sth

2.3.1.183: phosphinothricin acetyltransferase - BRENDA

The bar Gene as Selectable and Screenable Marker in Plant Engineering …

WebMar 9, 2024 · Plasmid pGL2, bearing the hygromycin phosphotransferase gene ( hph) coding sequence under control of the CaMV35S promoter, is used for hygromycin selection [ 12 ]. Plasmid DNA is isolated using a modified cetyltriethylammoniumbromide (CTAB) extraction method [ 13 ]. Plasmid DNA is dissolved in water and stored at −20 °C. WebApr 3, 2024 · Resveratrol-enriched rice (RR) includes the stilbene synthase gene for resveratrol synthesis and the phosphinothricin-N-acetyltransferase gene for glufosinate tolerance.To investigate unintended effects resulting from RR’s genetically modified chemical composition, 56 polar and nonpolar secondary metabolites were analyzed with … shared office space wilmington deWebexpression cassette encodes a phosphinothricin acetyltransferase gene from Streptomyces . viridochromogenes . that is codon-optimized for expression in corn. Method for conferring pool table overland park

"WebApr 11, 2024 · The Animal and Plant Health Inspection Service (APHIS) issued a final rule, published in the Federal Register on May 18, 2024 (85 FR 29790-29838, Docket No. APHIS-2024-0034) \1\, revising 7 CFR part 340; however, the final rule is being implemented in phases. The new Regulatory Status Review (RSR) process, which replaces the petition for ... " - Phosphinothricin acetyltransferase gene

Phosphinothricin acetyltransferase gene

Utility of the pat gene as a selectable marker gene in production of …

WebInactivates phosphinothricin (PPT) by transfer of an acetyl group from acetyl CoA. Can also acetylate demethylphosphinothricin but not PTT or glutamate. This enzyme is an effector … WebFeb 16, 2016 · Wohlleben, W. et al. Nucleotide sequence of the phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes Tü494 and its expression in Nicotiana tabacum. Gene 70 , 25–37 (1988).

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WebJun 15, 1992 · Cloning of a phosphinothricin N- acetyltransferase gene from Streptomyces viridochromogenes Tü494 and its expression in Streptomyces lividans and Escherichia coli Gene (1988) P.G. Reddy et al. Cloning and expression in Escherichia coli of an esterase-coding gene from the oil-degrading bacterium Acinobacter calcoaceticus RAG-1 Gene … WebNov 27, 2024 · Bialaphos resistance (BAR) and phosphinothricin acetyltransferase (PAT) genes, which convey resistance to the broad-spectrum herbicide phosphinothricin (also …

WebApr 11, 2024 · the gene that codes for the phosphinothricin acetyltransferase protein responsible for tolerance to glufosinate-ammonium herbicides. DP23211 corn also contains the gene that encodes for the phosphomannose isomerase protein, which is used as a selectable marker. The petition states that DP23211 corn is unlikely to pose a WebPhosphinothricin acetyltransferase (PAT) assays confirmed the expression of the bar gene in plants obtained from PPT-resistant calli. The only exceptions were two plants obtained from the same callus that had multiple copies of the bar gene integrated into their genomes. The transgenic status of the plants was varified by Southern blot analysis.

WebGene: pat. Gene Source: Streptomyces viridochromogenes. Product: phosphinothricin N-acetyltransferase (PAT) enzyme. Function: eliminates herbicidal activity of glufosinate … WebApr 11, 2024 · Pioneer Hi-Bred International (Pioneer) has submitted a petition (APHIS Petition Number 20–203–01p) to APHIS seeking a determination of nonregulated status for DP23211 maize (corn), which was developed using genetic engineering for insect resistance to western corn rootworm and contains the gene that codes for the phosphinothricin ...

WebAbstract. Insertion of the gene encoding phosphinothricin acetyltransferase (PAT) has resulted in cotton plants resistant to the herbicide glufosinate. However, the lower …

WebFeb 1, 2014 · The genes encoding for bialaphos resistance (phosphinothricin acetyltransferase), originally cloned from bialaphos producing streptomycetes [ 3, 56, 59, 63 ], have been used as selection markers in plant biology and engineering [ 11 ]. shared office space windsor ontarioWebThe chimeric hygromycin phosphotransferase gene ( hph) and phosphinothricin acetyltransferase gene ( bar) have been used as selectable markers. The integration and expression of an hph gene in … shared office space tacomaWebDroege, W.; Broer, I.; Puehler, A.: Transgenic plants containing the phosphinothricin-N-acetyltransferase gene metabolize the herbicide l-phosphinothricin (glufosinate) … pool table parts stick tipsWebWohlleben W, Arnold W, Broer I, Hillemann D, Strauch E, Pühler A (1988) Nucleotide sequence of the phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes TÜ494 and its expression in Nicotiana tabacum. Gene … pool table pad hard cover for diningWebmechanism of Agrobacterium-mediated gene transfer has been widely utilized to genetically modify mono-cots and dicots (Hansen and Wright, 1999; Fukuoka et al., 2000). Agrobacterium tumefaciensis a soil-borne pathogen that has the capacity to infect plants and transfer a segment of DNA (T-DNA) from its tumor-inducing (Ti) plasmid to the plant … shared one drive accountWebApr 23, 2012 · The phosphinothricin expression cassette consists of the NOS promoter (promoter of the A. tumefaciens nopaline synthase gene), the pat cds (phosphinothricin acetyltransferase gene from Streptomyces viridochromogenes), and the NOS terminator (terminator of the A. tumefaciens nopaline synthase gene). pool table party rental near meWebThe gene encodes phosphinothricin acetyltransferase, which N -acetylates phosphinothricin using acetyl-CoA. Sign in to download full-size image Figure 6.1. Overview of biosynthetic pathways of a number of phosphonate and phosphinate natural products. The first two steps of phosphonate biosynthesis (boxed) are conserved for most known … share donation agreement