Webthe lack of a d-cell-specific Cre line, we used Pdx1-CreER (Gu et al. 2002) mice, in which Cre recombinase is active in both b and d cells upon induction by tamoxifen (Guz et al. 1995; Serup et al. 1995; Schaffer et al. 2011). We reasoned that because Hhex is not expressed in b cells, 2014 Zhang et al. This article is distributed exclusively by ... WebFeb 4, 2014 · Pdx1 is a homeobox-containing transcription factor that plays a key role in pancreatic development and adult β cell function. In this study, we traced the fate of adult β cells after Pdx1 deletion. As expected, β-cell-specific removal of Pdx1 resulted in severe hyperglycemia within days.
Nkx6 Transcription Factors and Ptf1a Function as Antagonistic …
WebOct 10, 2015 · To determine the fate of Sox9-or Pdx1-expressing cells in the foregut endoderm, we performed lineage tracing in embryos carrying the Rosa26 mTomato/mGFP (R26 mT/mG) reporter allele and an inducible form of Cre-recombinase, CreER, driven by either Sox9 or Pdx1 regulatory sequences. In these mice, tamoxifen administration to … WebDec 1, 2003 · To determine whether these early Pdx1+ cells represent a unique population with respect to Notch responsiveness, we used a transgenic strain, Pdx1-CreER ( 1 ), in which the Pdx1 promoter drives expression of a fusion protein between Cre recombinase and a TM-dependent estrogen receptor (ER™). precinct 2 dallas county
PDX1 - Wikipedia
Web037551 - NOD.pdx1 [PB] CreER [TM] Strain Details. Technical Information Scientists. Overview. http://genesdev.cshlp.org/content/28/8/829.long WebThe Pdx1-CreERTM (−) mice ( C) or the mice without ( A) TAM injection are shown as negative controls. Scale bar, 50 μm. 2.2. Assessment of Glucose Tolerance in A0B Δpanc and A0B2 Mice The body weights of the mice in each group were recorded from 4 weeks to 16 weeks post-TAM injection. scooter up a ramp