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Pdx1-creer

Webthe lack of a d-cell-specific Cre line, we used Pdx1-CreER (Gu et al. 2002) mice, in which Cre recombinase is active in both b and d cells upon induction by tamoxifen (Guz et al. 1995; Serup et al. 1995; Schaffer et al. 2011). We reasoned that because Hhex is not expressed in b cells, 2014 Zhang et al. This article is distributed exclusively by ... WebFeb 4, 2014 · Pdx1 is a homeobox-containing transcription factor that plays a key role in pancreatic development and adult β cell function. In this study, we traced the fate of adult β cells after Pdx1 deletion. As expected, β-cell-specific removal of Pdx1 resulted in severe hyperglycemia within days.

Nkx6 Transcription Factors and Ptf1a Function as Antagonistic …

WebOct 10, 2015 · To determine the fate of Sox9-or Pdx1-expressing cells in the foregut endoderm, we performed lineage tracing in embryos carrying the Rosa26 mTomato/mGFP (R26 mT/mG) reporter allele and an inducible form of Cre-recombinase, CreER, driven by either Sox9 or Pdx1 regulatory sequences. In these mice, tamoxifen administration to … WebDec 1, 2003 · To determine whether these early Pdx1+ cells represent a unique population with respect to Notch responsiveness, we used a transgenic strain, Pdx1-CreER ( 1 ), in which the Pdx1 promoter drives expression of a fusion protein between Cre recombinase and a TM-dependent estrogen receptor (ER™). precinct 2 dallas county https://tomanderson61.com

PDX1 - Wikipedia

Web037551 - NOD.pdx1 [PB] CreER [TM] Strain Details. Technical Information Scientists. Overview. http://genesdev.cshlp.org/content/28/8/829.long WebThe Pdx1-CreERTM (−) mice ( C) or the mice without ( A) TAM injection are shown as negative controls. Scale bar, 50 μm. 2.2. Assessment of Glucose Tolerance in A0B Δpanc and A0B2 Mice The body weights of the mice in each group were recorded from 4 weeks to 16 weeks post-TAM injection. scooter up a ramp

PDX1 Gene - GeneCards PDX1 Protein PDX1 Antibody

Category:Tamoxifen-Independent Recombination in the RIP-CreER Mouse

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Pdx1-creer

A gene regulatory network cooperatively controlled by Pdx1 and …

WebJun 1, 2009 · Interestingly, in Pdx1 ΔC/ΔC mice, there was a global reduction in endocrine lineages, although β and α cells were more severely affected (Figure 4C). Only β cell area … WebThe unbridled insulin secretion in mutants stemmed from Hhex deficiency rather than ectopic expression of Cre in the β cell from the Pdx1-CreER transgenes, whose lack of effect on insulin secretion has been validated in several reports (Lee et al. 2011; Schaffer et al. 2011; Gao et al. 2014).

Pdx1-creer

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http://genesdev.cshlp.org/content/28/8/829.full.pdf WebTg (Pdx1-cre/Esr1*)#Dam. Mutation details : The sequence encoding a tamoxifen-inducible form of a cre recombinase/estrogen receptor fusion protein was adjoined to the Ipf1 …

WebThe F1 progeny were heterozygous for Prmt5 (Prmt5+/l). Subsequent breeding between F1 mice resulted in a population of progeny that included the desired genotype Prmt5l/l. Then, Pdx1-CreER; Prmt5l/l mice were generated by intercrossing Pdx1-CreER mice with Prmt5l/l mice. (B-C) The genotypes were verified by PCR using tail DNA. WebDec 12, 2024 · We then crossed these mice with β-cell-specific Pdx1-CreER mice carrying a transgene placing the tamoxifen-inducible cyclic recombinase - estrogen receptor ( Cre-ER) fusion construct under the...

WebDec 1, 2003 · To determine whether these early Pdx1 + cells represent a unique population with respect to Notch responsiveness, we used a transgenic strain, Pdx1-CreER , in which … WebPDX1; pancreatic and duodenal homeobox 1: Aliases: GSF, IPF1, IUF1, IDX-1, MODY4, PDX-1, STF-1, PAGEN1 : Location: 13q12.2: Summary: The protein encoded by this gene is a …

WebNov 28, 2008 · The β-cell–to-acinar transdifferentiation was observed not only when the caAkt mutant was expressed in β-cells during development (Rip-cre;caAkt), but also in mature β-cells from 4-week-old mice (Pdx1 CreER;caAkt). These results also demonstrate the plasticity of postnatal mature β-cells.

WebNov 16, 2013 · The inducible Pdx1-CreER;Vegfa fl/fl line is an effective tool for VEGF-A inactivation in adult pancreatic islets. A: Time line of VEGF-A inactivation in Pdx1-Cre;Vegfa fl/fl and Pdx1 PB-CreER;Vegfa fl/fl mice. Effects of early VEGF-A inactivation in Pdx1-Cre;Vegfa fl/fl line were analyzed at e14.5, P1 and 2 months (mo) and 6 months of age. scooter urbanWebOct 1, 2015 · To determine the fate of Sox9-or Pdx1-expressing cells in the foregut endoderm, we performed lineage tracing in embryos carrying the Rosa26 mTomato/mGFP (R26 mT/mG) reporter allele and an inducible form of Cre-recombinase, CreER, driven by either Sox9 or Pdx1 regulatory sequences. In these mice, tamoxifen administration to … scooter university of floridaWebPDX1 (pancreatic and duodenal homeobox 1), also known as insulin promoter factor 1, is a transcription factor in the ParaHox gene cluster. In vertebrates, Pdx1 is necessary for … scooter uprightscooter uphillWebJun 15, 2010 · We utilized the Pdx1 promoter to misexpress Nkx6.1 or Nkx6.2 ( Figures 2 A and 2H ). As predicted, Pdx1-Nkx6.1 or Pdx1-Nkx6.2 embryos expressed Nkx6.1 or Nkx6.2, respectively, throughout the entire pancreatic epithelium, including the presumptive tip domain ( Figures 2 C, 2E, 2G, 2J, 2L, and 2N). scooter urban artWebMar 21, 2024 · GeneCards Summary for PDXDC1 Gene. PDXDC1 (Pyridoxal Dependent Decarboxylase Domain Containing 1) is a Protein Coding gene. Diseases associated with … precinct 2 corpus christi texasWebNational Center for Biotechnology Information precinct2gether inc